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osteogenic differentiation media  (Lonza)


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    Lonza osteogenic differentiation media
    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent <t>osteogenic</t> differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
    Osteogenic Differentiation Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteogenic+differentiation+media/osteogenic+differentiation+medium/pmc12015357-316-22-25
    Average 90 stars, based on 1 article reviews
    osteogenic differentiation media - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing"

    Article Title: Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing

    Journal: NPJ Regenerative Medicine

    doi: 10.1038/s41536-025-00408-z

    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent osteogenic differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
    Figure Legend Snippet: A Representative overlayed images depicting RUNX2 expression in MSCs that underwent osteogenic differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.

    Techniques Used: Expressing

    Related Articles

    Cell Culture:

    Article Title: Impact of Vancomycin Treatment on Human Mesenchymal Stromal Cells During Osteogenic Differentiation.
    Article Snippet: Background: Vancomycin is frequently applied locally to the operative site during foot and ankle procedures to help prevent infection.. Although the efficacy of locally applied vancomycin has been demonstrated in spine surgery, there is no consensus on dosing and indication within foot and ankle surgery.. Osteogenic differentiation of human mesenchymal stromal cells (hMSCs) is key to healing of both fractures and arthrodesis.

    Article Title: Multivalent Biomaterial Platform to Control the Distinct Arterial Venous Differentiation of Pluripotent Stem Cells
    Article Snippet: .. Alizarin red and alkaline phosphatase analysis To evaluate osteogenic potential of arterial and venous Flk1 populations, cells were cultured under osteogenic differentiation media (Lonza) for seven days. ..

    Article Title: Multivalent Biomaterial Platform to Control the Distinct Arterial Venous Differentiation of Pluripotent Stem Cells
    Article Snippet: .. To evaluate osteogenic potential of arterial and venous Flk1 populations, cells were cultured under osteogenic differentiation media (Lonza) for seven days. ..

    other:

    Article Title: Gain of CXCR7 function with mesenchymal stem cell therapy ameliorates experimental arthritis via enhancing tissue regeneration and immunomodulation
    Article Snippet: Osteogenic and chondrogenic differentiation media were purchased from Lonza.

    Transfection:

    Article Title: Differential BMP Signaling Mediates the Interplay Between Genetics and Leaflet Numbers in Aortic Valve Calcification
    Article Snippet: .. One day after seeding, the cells were transfected with siRNAs for 24 hours, and then the culture media was replaced with osteogenic differentiation media (Lonza, Basel, Switzerland) for 72 hours with or without human recombinant Noggin (500 ng/ml). ..

    Recombinant:

    Article Title: Differential BMP Signaling Mediates the Interplay Between Genetics and Leaflet Numbers in Aortic Valve Calcification
    Article Snippet: .. One day after seeding, the cells were transfected with siRNAs for 24 hours, and then the culture media was replaced with osteogenic differentiation media (Lonza, Basel, Switzerland) for 72 hours with or without human recombinant Noggin (500 ng/ml). ..



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    Lonza osteogenic differentiation media
    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent <t>osteogenic</t> differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
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    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent <t>osteogenic</t> differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
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    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent <t>osteogenic</t> differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
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    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent <t>osteogenic</t> differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.
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    <t>HUCMSCs</t> ameliorated OVA-induced airway inflammation in mice through migrasomes. A HUCMSCs of wild type and TSPAN4-KD were stained with 1 µg/ml WGA (Alexa 488) and observed under confocal microscopy. Scale bar = 10 μm. B Western blot analysis of TSPAN4 expression in hUCMSCs of wild type and TSPAN4-KD. C Experimental protocol for the development of murine allergic asthma model. D Representative images of lung sections stained with HE (up) and PAS (down) from each group are presented (black bar = 100 μm). E The inflammatory infiltration and goblet cell hyperplasia were quantified by HE (left) and PAS (right) scores ( n = 6). F Statistical analysis was conducted on the total inflammatory cells (left) and eosinophils (right) in the BALF ( n = 6). Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was performed on the data. * P < 0.05, ** P < 0.01, *** P < 0.001. Full-length blots are presented in supplemental Figure
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    Image Search Results


    A Representative overlayed images depicting RUNX2 expression in MSCs that underwent osteogenic differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.

    Journal: NPJ Regenerative Medicine

    Article Title: Combinatorial extracellular matrix tissue chips for optimizing mesenchymal stromal cell microenvironment and manufacturing

    doi: 10.1038/s41536-025-00408-z

    Figure Lengend Snippet: A Representative overlayed images depicting RUNX2 expression in MSCs that underwent osteogenic differentiation on the tissue chips. Red: RUNX2; Blue: Hoechst. Scale Bar: 100 µm. B Dot plot of RUNX2%+ MSCs as a function of stiffness. Each dot represents a metric for a single image on a specific ECM, and the red line represents mean. C Heatmap of scaled RUNX2%+ MSCs on different ECM combinations and stiffness. The ECMs are ranked from highest to lowest based on their averages across stiffnesses. D Venn diagram of top ECMs for osteogenesis for each stiffness, with common ECMs across two stiffnesses and three stiffnesses highlighted. E Bar plot of estimated coefficients in the linear regression model of osteogenesis as a function of individual ECM components, ranked from high to low. F Bar plot of difference in average RUNX2%+ MSCs when a certain ECM combination is present versus absent. Green: Significant combination ( P < 0.05); Orange: Close to Significant ( P < 0.1). Data presented here corresponds to at least three biological replicates. * denotes P < 0.05.

    Article Snippet: MSCs were seeded at 2 × 10 4 /cm 2 for each well and allowed to grow to confluency before changing to osteogenic differentiation media (Lonza).

    Techniques: Expressing

    HUCMSCs ameliorated OVA-induced airway inflammation in mice through migrasomes. A HUCMSCs of wild type and TSPAN4-KD were stained with 1 µg/ml WGA (Alexa 488) and observed under confocal microscopy. Scale bar = 10 μm. B Western blot analysis of TSPAN4 expression in hUCMSCs of wild type and TSPAN4-KD. C Experimental protocol for the development of murine allergic asthma model. D Representative images of lung sections stained with HE (up) and PAS (down) from each group are presented (black bar = 100 μm). E The inflammatory infiltration and goblet cell hyperplasia were quantified by HE (left) and PAS (right) scores ( n = 6). F Statistical analysis was conducted on the total inflammatory cells (left) and eosinophils (right) in the BALF ( n = 6). Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was performed on the data. * P < 0.05, ** P < 0.01, *** P < 0.001. Full-length blots are presented in supplemental Figure

    Journal: Stem Cell Research & Therapy

    Article Title: Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice

    doi: 10.1186/s13287-025-04145-4

    Figure Lengend Snippet: HUCMSCs ameliorated OVA-induced airway inflammation in mice through migrasomes. A HUCMSCs of wild type and TSPAN4-KD were stained with 1 µg/ml WGA (Alexa 488) and observed under confocal microscopy. Scale bar = 10 μm. B Western blot analysis of TSPAN4 expression in hUCMSCs of wild type and TSPAN4-KD. C Experimental protocol for the development of murine allergic asthma model. D Representative images of lung sections stained with HE (up) and PAS (down) from each group are presented (black bar = 100 μm). E The inflammatory infiltration and goblet cell hyperplasia were quantified by HE (left) and PAS (right) scores ( n = 6). F Statistical analysis was conducted on the total inflammatory cells (left) and eosinophils (right) in the BALF ( n = 6). Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was performed on the data. * P < 0.05, ** P < 0.01, *** P < 0.001. Full-length blots are presented in supplemental Figure

    Article Snippet: HUCMSCs and hUCMSC TSPAN4−KD were cultured in OriCellTM hUCMSCs osteogenic differentiation media (Cyagen) as described by manufacturer.

    Techniques: Staining, Confocal Microscopy, Western Blot, Expressing